• 1 蘭州大學(xué)附屬天浩醫(yī)院(甘肅蘭州 730060);;
  • 2 甘肅省人民醫(yī)院;;
  • 3 蘭州大學(xué)第二醫(yī)院呼吸內(nèi)科;

目的    探討 霧化吸入前列腺素 E1 ( PGE1 )對脂多糖(LPS)所致大鼠急性肺損傷(ALI)模型Th1/Th2的影響。方法  尾靜脈注射LPS復(fù)制大鼠ALI模型,PGE1霧化入寬大玻璃瓶內(nèi)讓大鼠自由吸入,用ELISA法檢測大鼠外周血和支氣管肺泡灌洗液(BALF)中干擾素γ(IFN-γ)、白細(xì)胞介素4(IL-4)的水平及IFN-γ/IL-4比值。結(jié)果  LPS組外周血及BALF中IFN-γ/IL-4比值較生理鹽水對照組(NS組)顯著升高(P lt;0.01),PGE1組外周血IFN-γ/IL-4(Th1/Th2)比值在6、12及24 h時(shí)間點(diǎn)顯著低于LPS組(P lt;0.01),而BALF中各時(shí)間點(diǎn)IFN-γ/IL-4(Th1/Th2)比值顯著低于LPS組(P lt;0.01)。結(jié)論  霧化吸入PGE1可使LPS所致大鼠ALI失衡的Th1/Th2趨于平衡,減輕LPS所致大鼠ALI。

引用本文: 付明生 ,肖曉輝,萬毅新. 吸入前列腺素E1對脂多糖所致大鼠急性肺損傷模型Th1/Th2的影響. 中國呼吸與危重監(jiān)護(hù)雜志, 2008, 08(1): 47-51. doi: 復(fù)制

1. 1 Tamura DY , Moore EE ,Partrick DA,et al.Prostaglandin E1 attenuates cytotoxic mechanisms of primed neutrophils.Shock,1998,9:171-176.
2. Zedler S,Bone RC,Baue AE,et al.T-cell reactivity and its predictive role in immuno- suppression after burns.Crit Care Med,1999,27:66-72.
3. 馮英凱,徐劍鋮,楊慶華,等.內(nèi)毒素肺損傷大鼠Th1、Th2細(xì)胞凋亡的變化及其作用探討.重慶醫(yī)學(xué),2005,34:74-77.
4. 韋俊,梁自乾,孫秀鋒.早期霧化吸入前列腺素E1對大鼠煙霧吸入性肺損傷的保護(hù)作用.廣西醫(yī)科大學(xué)學(xué)報(bào),2006,23:266-269.
5. Marsh CB,Wewer MD.The pathogenesis of sepsis:factor that modulate the response to gram-negative bacterial infection.Clin Chest Med,1996,17:183-197.
6. Li B,Toumier C,Divis RJ,et al.Regulation of IL-4 expression by the transcription factor Jun B during T helper cell differentiation.EMBO J,1999,18:420-432.
7. Traber DL,Linares HA,Herndon DN.The pathophysiology of inhalation injury a review.Burns,1998,14:357.
8. Wajima I,Shiga T,Yoshikawa T,et al.Intravenous alprostadil,an analog of prostaglandin E1,prenents thiamyal fentanyl induced bronchoconstriction in humans.Anesth Analg,2003,97:456-460.
9. De perrot M,F(xiàn)ischer S,Liu M,et al.Prostaglandin E1 protects lung 30 transplants from ischemia-reperfusion injury:a shift from pro to anti inflammatory cytokines.Transplantation,2001,72:1505-1512.
10. Chopra J,Webster RO.PGE1 inhibits neutrophil adherence and neutrophil-mediared injury to cultured endothelial cells.Am Rev Respir Dis,1998,138:915.
11. Tamura DY,Moore EE,Partrick DA,et al.Prostaglandin E1 attenuates cytotoxic mechanisms of primed neutrophils.Shock,1998,9:171-176.
12. Bao S,Wang Y,Sweeney P,et al.Keratinocyte growth factor induces Akt kinase activity and inhibits Fas-mediated apoptosis in A549 lung epithelial cells.Am J Physiol Lung Cell Mol Physiol,2005,288:L36-L42.
13. 岳子勇,宋春雨,李文志,等.霧化吸入PGE1對急性肺損傷豬氧合及肺內(nèi)分流的影響.中華麻醉醫(yī)學(xué)雜志,2006,26:435-438. 14 Schmid -Weber CB,Alexander SI,Henault L E,et al.IL–4 enhances IL-10 gene expression inmurine TH2 cells in the absence of TCR engagement [J].Immunol,1999,162:238.李洪霞,張進(jìn)川,趙亞力等,地塞米松對急性肺損傷大鼠炎癥介質(zhì)/抗炎介質(zhì)表達(dá)的影響.中國呼吸與危重監(jiān)護(hù)雜志.2005,4(1):65-68.
  1. 1. 1 Tamura DY , Moore EE ,Partrick DA,et al.Prostaglandin E1 attenuates cytotoxic mechanisms of primed neutrophils.Shock,1998,9:171-176.
  2. 2. Zedler S,Bone RC,Baue AE,et al.T-cell reactivity and its predictive role in immuno- suppression after burns.Crit Care Med,1999,27:66-72.
  3. 3. 馮英凱,徐劍鋮,楊慶華,等.內(nèi)毒素肺損傷大鼠Th1、Th2細(xì)胞凋亡的變化及其作用探討.重慶醫(yī)學(xué),2005,34:74-77.
  4. 4. 韋俊,梁自乾,孫秀鋒.早期霧化吸入前列腺素E1對大鼠煙霧吸入性肺損傷的保護(hù)作用.廣西醫(yī)科大學(xué)學(xué)報(bào),2006,23:266-269.
  5. 5. Marsh CB,Wewer MD.The pathogenesis of sepsis:factor that modulate the response to gram-negative bacterial infection.Clin Chest Med,1996,17:183-197.
  6. 6. Li B,Toumier C,Divis RJ,et al.Regulation of IL-4 expression by the transcription factor Jun B during T helper cell differentiation.EMBO J,1999,18:420-432.
  7. 7. Traber DL,Linares HA,Herndon DN.The pathophysiology of inhalation injury a review.Burns,1998,14:357.
  8. 8. Wajima I,Shiga T,Yoshikawa T,et al.Intravenous alprostadil,an analog of prostaglandin E1,prenents thiamyal fentanyl induced bronchoconstriction in humans.Anesth Analg,2003,97:456-460.
  9. 9. De perrot M,F(xiàn)ischer S,Liu M,et al.Prostaglandin E1 protects lung 30 transplants from ischemia-reperfusion injury:a shift from pro to anti inflammatory cytokines.Transplantation,2001,72:1505-1512.
  10. 10. Chopra J,Webster RO.PGE1 inhibits neutrophil adherence and neutrophil-mediared injury to cultured endothelial cells.Am Rev Respir Dis,1998,138:915.
  11. 11. Tamura DY,Moore EE,Partrick DA,et al.Prostaglandin E1 attenuates cytotoxic mechanisms of primed neutrophils.Shock,1998,9:171-176.
  12. 12. Bao S,Wang Y,Sweeney P,et al.Keratinocyte growth factor induces Akt kinase activity and inhibits Fas-mediated apoptosis in A549 lung epithelial cells.Am J Physiol Lung Cell Mol Physiol,2005,288:L36-L42.
  13. 13. 岳子勇,宋春雨,李文志,等.霧化吸入PGE1對急性肺損傷豬氧合及肺內(nèi)分流的影響.中華麻醉醫(yī)學(xué)雜志,2006,26:435-438. 14 Schmid -Weber CB,Alexander SI,Henault L E,et al.IL–4 enhances IL-10 gene expression inmurine TH2 cells in the absence of TCR engagement [J].Immunol,1999,162:238.李洪霞,張進(jìn)川,趙亞力等,地塞米松對急性肺損傷大鼠炎癥介質(zhì)/抗炎介質(zhì)表達(dá)的影響.中國呼吸與危重監(jiān)護(hù)雜志.2005,4(1):65-68.
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