目的 研究肝細(xì)胞肝癌細(xì)胞中增殖細(xì)胞核抗原（PCNA）/凋亡細(xì)胞比值與肝細(xì)胞肝癌分化程度的關(guān)系。
方法 應(yīng)用原位末端標(biāo)記法（TUNEL）及免疫組化雙染色技術(shù)，對35例肝細(xì)胞肝癌石蠟包埋組織進(jìn)行檢測。
結(jié)果 不同分化程度癌組織中細(xì)胞凋亡和PCNA的陽性率不同。分化程度高，細(xì)胞凋亡陽性率高、PCNA陽性率偏低； 分化程度低，細(xì)胞凋亡陽性率偏低、PCNA陽性率高。隨分化程度的降低，PCNA/凋亡細(xì)胞的比值顯著增加，二者有良好的相關(guān)性。
結(jié)論 PCNA/凋亡細(xì)胞的比值可作為評估肝細(xì)胞肝癌分化程度的指標(biāo)。
引用本文： 何勇,周峻,竇科峰. 細(xì)胞凋亡及增殖細(xì)胞核抗原雙染色在肝細(xì)胞肝癌組織中的研究. 中國普外基礎(chǔ)與臨床雜志, 2001, 8(4): 249-251. doi: 復(fù)制
版權(quán)信息： ?四川大學(xué)華西醫(yī)院華西期刊社《中國普外基礎(chǔ)與臨床雜志》版權(quán)所有，未經(jīng)授權(quán)不得轉(zhuǎn)載、改編
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- 4. Miyachi K. Autoantibody to a nuclear antigen in proliferating cells 〔Ｊ〕. Journal of Immunology, 1978; 121(10)∶2228.
- 5. Mathews MB, Bernstein RM, Franza BR Jr, et al. Identity of the proliferating cell nuclear antigen and cyclin 〔Ｊ〕. Nature, 1984; 309(5966)∶374.
- 6. Tuusa J, Uitto L, Syvaoja JE, Human DNA polymerase epsilon is expressed during cell proliferation in a manner characteristic of replicative DNA polymerases 〔Ｊ〕. Nucleic Acid Res, 1995; 23(12)∶2178.
- 7. Irene OL, Edward CS, Sheung T, et al. Prognostic significance of proliferating cell nuclear antigen expression in hepatocellular carcinoma 〔Ｊ〕. Cancer, 1994; 73(9)∶2268.
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- 9. Hino N, Higashi T, Nouso K, et al. Apoptosis and proliferation of human hepatocellular carcinoma 〔Ｊ〕. Liver, 1996; 16(2)∶123.