羊水干细胞在再生医学的研究进展_《中国修复重建外科杂志》

作者：

李春 ¹ , 李巍松 ¹ , 刘虎 ¹ , 汪洁 ²

1安徽医科大学第一附属医院小儿外科（合肥，230022）;;
2安徽医科大学第四附属医院皮肤科;

关键词：

羊水干细胞多向分化再生医学组织工程

DOI：

10.7507/1002-1892.20130275

视频：

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摘要 全文 图表 视频 参考文献 施引文献 补充材料

目的对羊水干细胞（amniotic fluid-derived stem cells，AFSCs）的研究进展及在再生医学等领域的发展进行综述，探讨今后AFSCs研究的相关问题。方法查阅近年来AFSCs的相关文献，对AFSCs在再生医学领域研究的成果及其基础与临床研究展望进行综述。结果目前研究已表明AFSCs具有多向分化潜能，在组织工程或细胞替代治疗的研究中展现出广阔的应用前景。结论羊水取材简便，对母婴创伤小，无致瘤性，无伦理学方面的限制，使AFSCs极有希望成为再生医学、抗肿瘤等领域中理想的种子细胞，具有广泛研究价值。

引用本文： 李春,李巍松,刘虎,汪洁. 羊水干细胞在再生医学的研究进展. 中国修复重建外科杂志, 2013, 27(10): 1262-1266. doi: 10.7507/1002-1892.20130275 复制

1.	Torricelli F, Brizzi L, Bernabei PA, et al. Identification of hematopoietic progenitor cells in human amniotic fluid before the 12th week of gestation. Ital J Anat Embryol, 1993, 98(2): 119-126.
2.	Rosner M, Schipany K, Shanmugasundaram B, et al. Amniotic fluid stem cells: future perspectives. Stem Cells, 2012, 2012: 741810.
3.	Gundacker C, Dolznig H, Mikula M, et al. Amniotic fluid stem cell-based models to study the effects of genemutations and toxicants onmale germ cell formation. Asian J Androl, 2012, 14(2): 247-250.
4.	Rosner M, Dolznig H, Schipany K, et al. Human amniotic fluid stem cells as a model for functional studies of genes involved in human genetic diseases or oncogenesis. Oncotarget, 2011, 2(9): 705-712.
5.	Rosner M, Schipany K, Gundacker C, et al. Renal differentiation of amniotic fluid stem cells: perspectives for clinical application and for studies on specific human genetic diseases. Euro J Clin Invest, 2012, 42(6): 677-684.
6.	Da Sacco S, Sedrakyan S, Boldrin F, et al. Human amniotic fluid as a potential new source of organ specific precursor cells for future regenerative medicine applications. J Urol, 2010, 183(3): 1193-1200.
7.	Kang NH, Hwang KA, Kim YB, et al. Potential antitumor therapeutic strategies of human amniotic membrane and amniotic fluid-derived stem cells. Cancer Gene Ther, 2012, 19(8): 517-522.
8.	Cabral AC, Angelo PC, Leite HV, et al. Isolation differentiation and biochemical aspects of amniotic fluid stem cell. Rev Assoc Med Bras, 2008, 54(6): 489-493.
9.	Soncini M, Vertua E, Gibelli L, et al. Isolation and characterization of mesenchymal cells from human fetal membranes. J Tissue Eng Regen Med, 2007, 1(4) : 296-305.
10.	Atala A, Bauer SB, Soker S, et al. Tissue-engineered autologous bladders for patients needing cystoplasty. Lancet, 2006, 367(9518): 1241-1246 .
11.	Raya-Rivera A, Esquiliano DR, Yoo JJ, et al. Tissue-engineered autologous urethras for patients who need reconstruction: an observational study. Lancet, 2011, 377(9772): 1175-1182.
12.	Yang DY, Sheu ML, Su HL, et al. Dual regeneration of muscle and nerve by intravenous administration of human amniotic fluid-derived mesenchymal stem cells regulated by stromal cell-derived factor-1α in a sciatic nerve injury model. J Neurosurg, 2012, 116(6): 1357-1367.
13.	Joo S, Kol K, Atala A, et al. Amniotic fluid-derived stem cells in regenerative medicine research. Arch Pharm Res, 2012, 35(2): 271-280.
14.	Prasongchean W, Bagni M, Calzarossa C, et al. Amniotic fluid stem cells increase embryo survival following injury. Stem Cells, 2012, 21(5): 675-688.
15.	De Coppi P, Callegari A, Chiavegato A, et al. Amniotic fluid and bone marrow derived mesenchymal stem cells can be converted to smooth muscle cells in the cryo-injured rat bladder and prevent compensatory hypertrophy of surviving smooth muscle cells. J Urol, 2007, 177(1): 369-376.
16.	Lee WY, Wei HJ, Lin WW, et al. Enhancement of cell retention and functional benefits in myocardial infarction using human amniotic-fluid stemcell bodies enriched with endogenous ECM. Biomaterials, 2011, 32(24): 5558-5567.
17.	Mirabella T, Cilli M, Carlone S, et al. Amniotic liquid derived stem cells as reservoir of secreted angiogenic factors capable of stimulating neo-arteriogenesis in an ischemic model. Biomaterials, 2011, 32(15): 3689-3699.
18.	Carraro G, Perin L, Sedrakyan S, et al. Human amniotic fluid stem cells can integrate and differentiate into epithelial lung lineages. Stem Cells, 2008, 26(11): 2902-2911.
19.	Menasche P. Cell-based therapy for heart disease: a clinically oriented perspective. Mol Ther, 2009, 17(5): 758-766.
20.	Bollini S, Pozzobon M, Nobles M, et al. In vitro and in vivo cardiomyogenic differentiation of amniotic fluid stem cells. Stem Cell Rev, 2011, 7(2): 364-380.
21.	Yeh YC, Wei HJ, Lee WY, et al. Cellular cardiomyoplasty with human amniotic fluid stem cells: in vitro and in vivo studies. Tissue Eng Part A, 2010, 16(6): 1925-1936.
22.	Weber B, Zeisberger SM, Hoerstrup SP. Prenatally harvested cells for cardiovascular tissue engineering: fabrication of autologous implants prior to birth. Placenta, 2011, 32 Suppl 4: S316-S319.
23.	Schmidt D, Achermann J, Odermatt B, et al. Cryopreserved amniotic fluid-derived cells: a lifelong autologous fetal stem cell source for heart valve tissue engineering. J Heart Valve Dis, 2008, 17(4): 446-455.
24.	Parolini O, Soncini M, Evangelista M, et al. Amniotic membrane and amniotic fluid-derived cells: potential tools for regenerative medicine? Regen Med, 2009, 4(2): 275-291.
25.	Fauza DO, Marler JJ, Koka R, et al. Fetal tissue engineering: diaphragmatic replacement. J Pediatr Surg , 2001, 36(1): 146-151.
26.	Zheng YB, Zhang XH, Huang ZL, et al. Amniotic-fluid-derived mesenchymal stem cells overexpressing interleukin-1 receptor antagonist improve fulminant hepatic failure. PLoS One, 2012, 7(7): e41392.
27.	Perin L, Giuliani S, Jin D, et al. Renal differentiation of amniotic fluid stem cells. Cell Prolif, 2007, 40(6): 936-948.
28.	Sedrakyan S, Da Sacco S, Milanesi A, et al. Injection of amniotic fluid stem cells delays progression of renal fibrosis. J Am Soc Nephrol, 2012, 23(4): 661-673.
29.	Perin L, Sedrakyan S, Giuliani S, et al. Protective effect of human amniotic fluid stem cells in an immunodeficient mouse model of acute tubular necrosis. PLoS One, 2010, 5(2): e9357.
30.	Sun H, Feng K, Hu J, et al. Osteogenic differentiation of human amniotic fluid-derived stem cells induced by bone morphogenetic protein-7 and enhanced by nanofibrous scaffolds. Biomaterials, 2010, 31(6): 1133-1139.
31.	De Coppi P, Bartsch G Jr, Siddiqui MM, et al. Isolation of amniotic stem cell lines with potential for therapy. Nat Biotechnol, 2007, 25(1): 100-106.
32.	Peister A, Deutsch ER, Kolambkar Y, et al. Amniotic fluid stem cells produce robust mineral deposits on biodegradable scaffolds. Tissue Eng Part A, 2009, 15(10): 3129-3138.
33.	Dupont KM, Boerckel JD, Stevens HY, et al. Synthetic scaffold coating with adeno-associated virus encoding BMP2 to promote endogenous bone repair. Cell Tissue Res, 2012, 347(3): 575-588.
34.	Turner CG, Klein JD, Gray FL, et al. Craniofacial repair with fetal bone grafts engineered from amniotic mesenchymal stem cells. J Surg Res, 2012, 178(2): 785-790.
35.	Kolambkar YM, Peister A, Soker S, et al. Chondrogenic differentiation of amniotic fluid-derived stem cells. J Mol Histol, 2007, 38(5): 405-413.
36.	Iacono E, Merlo B, Pirrone A, et al. Effects of mesenchymal stem cells isolated from amniotic fluid and platelet-rich plasma gel on severe decubitus ulcers in a septic neonatal foal. Res Vet Sci, 2012, 93(3): 1439-1440.
37.	Kim K, Doi A, Wen B, et al. Epigenetic memory in induced pluripotent stem cells. Nature, 2010, 467(7313): 285-290.
38.	Fuchs C, Rosner M, Dolznig H, et al. Tuberin and PRAS40 are anti-apoptotic gatekeepers during early human amniotic fluid stem cell differentiation. Hum Mol Genet, 2012, 21(5): 1049-1061.
39.	Phermthai T, Odglun Y, Julavijitphong S, et al. A novel method to derive amniotic fluid stem cells for therapeutic purposes. BMC Cell Biol, 2010, 11: 79.
40.	Bitsika V, Roubelakis MG, Zagoura D, et al. Human amniotic fluid-derived mesenchymal stem cells as therapeutic vehicles: a novel approach for the treatment of bladder cancer. Stem Cells Dev, 2012, 21(7): 1097-1111.
41.	Kang NH, Hwang KA, Yi BR, et al. Human amniotic fluid-derived stem cells expressing cytosine deaminase and thymidine kinase inhibits the growth of breast cancer cells in cellular and xenograft mouse models. Cancer Gene Ther, 2012, 19(6): 412-419.
42.	Rosner M, Siegel N, Fuchs C, et al. Efficient siRNA-mediated prolonged gene silencing in human amniotic fluid stem cells. Nature Protocols, 2010, 5(6): 1081-1095.
43.	Manuelpillai U, Moodley Y, Borlongan CV, et al. Amniotic membrane and amniotic cells: Potential therapeutic tools to combat tissue inflammation and fibrosis? Placenta, 2011, 32 Suppl 4: S320-S325.
44.	罗宏武, 黄湘俊, 黄飞舟, 等. 人羊膜上皮细胞横向分化及脾内移植的初步研究. 中国修复重建外科杂志, 2011, 25(2): 144-148.
45.	涂长玉, 程敏, 李华锋, 等. 羊水穿刺超声定位的有关问题探讨. 中国优生优育, 2009, 15(1): 25-26.

1. Torricelli F, Brizzi L, Bernabei PA, et al. Identification of hematopoietic progenitor cells in human amniotic fluid before the 12th week of gestation. Ital J Anat Embryol, 1993, 98(2): 119-126.
2. Rosner M, Schipany K, Shanmugasundaram B, et al. Amniotic fluid stem cells: future perspectives. Stem Cells, 2012, 2012: 741810.
3. Gundacker C, Dolznig H, Mikula M, et al. Amniotic fluid stem cell-based models to study the effects of genemutations and toxicants onmale germ cell formation. Asian J Androl, 2012, 14(2): 247-250.
4. Rosner M, Dolznig H, Schipany K, et al. Human amniotic fluid stem cells as a model for functional studies of genes involved in human genetic diseases or oncogenesis. Oncotarget, 2011, 2(9): 705-712.
5. Rosner M, Schipany K, Gundacker C, et al. Renal differentiation of amniotic fluid stem cells: perspectives for clinical application and for studies on specific human genetic diseases. Euro J Clin Invest, 2012, 42(6): 677-684.
6. Da Sacco S, Sedrakyan S, Boldrin F, et al. Human amniotic fluid as a potential new source of organ specific precursor cells for future regenerative medicine applications. J Urol, 2010, 183(3): 1193-1200.
7. Kang NH, Hwang KA, Kim YB, et al. Potential antitumor therapeutic strategies of human amniotic membrane and amniotic fluid-derived stem cells. Cancer Gene Ther, 2012, 19(8): 517-522.
8. Cabral AC, Angelo PC, Leite HV, et al. Isolation differentiation and biochemical aspects of amniotic fluid stem cell. Rev Assoc Med Bras, 2008, 54(6): 489-493.
9. Soncini M, Vertua E, Gibelli L, et al. Isolation and characterization of mesenchymal cells from human fetal membranes. J Tissue Eng Regen Med, 2007, 1(4) : 296-305.
10. Atala A, Bauer SB, Soker S, et al. Tissue-engineered autologous bladders for patients needing cystoplasty. Lancet, 2006, 367(9518): 1241-1246 .
11. Raya-Rivera A, Esquiliano DR, Yoo JJ, et al. Tissue-engineered autologous urethras for patients who need reconstruction: an observational study. Lancet, 2011, 377(9772): 1175-1182.
12. Yang DY, Sheu ML, Su HL, et al. Dual regeneration of muscle and nerve by intravenous administration of human amniotic fluid-derived mesenchymal stem cells regulated by stromal cell-derived factor-1α in a sciatic nerve injury model. J Neurosurg, 2012, 116(6): 1357-1367.
13. Joo S, Kol K, Atala A, et al. Amniotic fluid-derived stem cells in regenerative medicine research. Arch Pharm Res, 2012, 35(2): 271-280.
14. Prasongchean W, Bagni M, Calzarossa C, et al. Amniotic fluid stem cells increase embryo survival following injury. Stem Cells, 2012, 21(5): 675-688.
15. De Coppi P, Callegari A, Chiavegato A, et al. Amniotic fluid and bone marrow derived mesenchymal stem cells can be converted to smooth muscle cells in the cryo-injured rat bladder and prevent compensatory hypertrophy of surviving smooth muscle cells. J Urol, 2007, 177(1): 369-376.
16. Lee WY, Wei HJ, Lin WW, et al. Enhancement of cell retention and functional benefits in myocardial infarction using human amniotic-fluid stemcell bodies enriched with endogenous ECM. Biomaterials, 2011, 32(24): 5558-5567.
17. Mirabella T, Cilli M, Carlone S, et al. Amniotic liquid derived stem cells as reservoir of secreted angiogenic factors capable of stimulating neo-arteriogenesis in an ischemic model. Biomaterials, 2011, 32(15): 3689-3699.
18. Carraro G, Perin L, Sedrakyan S, et al. Human amniotic fluid stem cells can integrate and differentiate into epithelial lung lineages. Stem Cells, 2008, 26(11): 2902-2911.
19. Menasche P. Cell-based therapy for heart disease: a clinically oriented perspective. Mol Ther, 2009, 17(5): 758-766.
20. Bollini S, Pozzobon M, Nobles M, et al. In vitro and in vivo cardiomyogenic differentiation of amniotic fluid stem cells. Stem Cell Rev, 2011, 7(2): 364-380.
21. Yeh YC, Wei HJ, Lee WY, et al. Cellular cardiomyoplasty with human amniotic fluid stem cells: in vitro and in vivo studies. Tissue Eng Part A, 2010, 16(6): 1925-1936.
22. Weber B, Zeisberger SM, Hoerstrup SP. Prenatally harvested cells for cardiovascular tissue engineering: fabrication of autologous implants prior to birth. Placenta, 2011, 32 Suppl 4: S316-S319.
23. Schmidt D, Achermann J, Odermatt B, et al. Cryopreserved amniotic fluid-derived cells: a lifelong autologous fetal stem cell source for heart valve tissue engineering. J Heart Valve Dis, 2008, 17(4): 446-455.
24. Parolini O, Soncini M, Evangelista M, et al. Amniotic membrane and amniotic fluid-derived cells: potential tools for regenerative medicine? Regen Med, 2009, 4(2): 275-291.
25. Fauza DO, Marler JJ, Koka R, et al. Fetal tissue engineering: diaphragmatic replacement. J Pediatr Surg , 2001, 36(1): 146-151.
26. Zheng YB, Zhang XH, Huang ZL, et al. Amniotic-fluid-derived mesenchymal stem cells overexpressing interleukin-1 receptor antagonist improve fulminant hepatic failure. PLoS One, 2012, 7(7): e41392.
27. Perin L, Giuliani S, Jin D, et al. Renal differentiation of amniotic fluid stem cells. Cell Prolif, 2007, 40(6): 936-948.
28. Sedrakyan S, Da Sacco S, Milanesi A, et al. Injection of amniotic fluid stem cells delays progression of renal fibrosis. J Am Soc Nephrol, 2012, 23(4): 661-673.
29. Perin L, Sedrakyan S, Giuliani S, et al. Protective effect of human amniotic fluid stem cells in an immunodeficient mouse model of acute tubular necrosis. PLoS One, 2010, 5(2): e9357.
30. Sun H, Feng K, Hu J, et al. Osteogenic differentiation of human amniotic fluid-derived stem cells induced by bone morphogenetic protein-7 and enhanced by nanofibrous scaffolds. Biomaterials, 2010, 31(6): 1133-1139.
31. De Coppi P, Bartsch G Jr, Siddiqui MM, et al. Isolation of amniotic stem cell lines with potential for therapy. Nat Biotechnol, 2007, 25(1): 100-106.
32. Peister A, Deutsch ER, Kolambkar Y, et al. Amniotic fluid stem cells produce robust mineral deposits on biodegradable scaffolds. Tissue Eng Part A, 2009, 15(10): 3129-3138.
33. Dupont KM, Boerckel JD, Stevens HY, et al. Synthetic scaffold coating with adeno-associated virus encoding BMP2 to promote endogenous bone repair. Cell Tissue Res, 2012, 347(3): 575-588.
34. Turner CG, Klein JD, Gray FL, et al. Craniofacial repair with fetal bone grafts engineered from amniotic mesenchymal stem cells. J Surg Res, 2012, 178(2): 785-790.
35. Kolambkar YM, Peister A, Soker S, et al. Chondrogenic differentiation of amniotic fluid-derived stem cells. J Mol Histol, 2007, 38(5): 405-413.
36. Iacono E, Merlo B, Pirrone A, et al. Effects of mesenchymal stem cells isolated from amniotic fluid and platelet-rich plasma gel on severe decubitus ulcers in a septic neonatal foal. Res Vet Sci, 2012, 93(3): 1439-1440.
37. Kim K, Doi A, Wen B, et al. Epigenetic memory in induced pluripotent stem cells. Nature, 2010, 467(7313): 285-290.
38. Fuchs C, Rosner M, Dolznig H, et al. Tuberin and PRAS40 are anti-apoptotic gatekeepers during early human amniotic fluid stem cell differentiation. Hum Mol Genet, 2012, 21(5): 1049-1061.
39. Phermthai T, Odglun Y, Julavijitphong S, et al. A novel method to derive amniotic fluid stem cells for therapeutic purposes. BMC Cell Biol, 2010, 11: 79.
40. Bitsika V, Roubelakis MG, Zagoura D, et al. Human amniotic fluid-derived mesenchymal stem cells as therapeutic vehicles: a novel approach for the treatment of bladder cancer. Stem Cells Dev, 2012, 21(7): 1097-1111.
41. Kang NH, Hwang KA, Yi BR, et al. Human amniotic fluid-derived stem cells expressing cytosine deaminase and thymidine kinase inhibits the growth of breast cancer cells in cellular and xenograft mouse models. Cancer Gene Ther, 2012, 19(6): 412-419.
42. Rosner M, Siegel N, Fuchs C, et al. Efficient siRNA-mediated prolonged gene silencing in human amniotic fluid stem cells. Nature Protocols, 2010, 5(6): 1081-1095.
43. Manuelpillai U, Moodley Y, Borlongan CV, et al. Amniotic membrane and amniotic cells: Potential therapeutic tools to combat tissue inflammation and fibrosis? Placenta, 2011, 32 Suppl 4: S320-S325.
44. 罗宏武, 黄湘俊, 黄飞舟, 等. 人羊膜上皮细胞横向分化及脾内移植的初步研究. 中国修复重建外科杂志, 2011, 25(2): 144-148.
45. 涂长玉, 程敏, 李华锋, 等. 羊水穿刺超声定位的有关问题探讨. 中国优生优育, 2009, 15(1): 25-26.

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羊水干细胞在再生医学的研究进展

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《中国修复重建外科杂志》

羊水干细胞在再生医学的研究进展

摘要 全文 图表 视频 参考文献 施引文献 补充材料

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